Extended Data Fig. 2: Nested downsampling of TRACERx data.
From: ImmuneLENS characterizes systemic immune dysregulation in aging and cancer
a, Correlation of T cell fraction at 60× coverage with samples downsampled to different coverage levels using nested downsampling. b, Example ImmuneLENS model outputs of the same sample at different downsampled coverage depths. c,d. Correlation of TCRB and TCRG T cell fractions at 60× coverage with samples downsampled to different coverage levels using nested downsampling. e, Correlation of circulating B cell fraction at 30× coverage with samples downsampled to different coverage levels using nested downsampling. Top: B cell fraction calculated without IGH haplotype correction. Bottom: B cell fraction calculated with IGH haplotype correction f, Correlation of infiltrating B cell fraction at 30× coverage with samples downsampled to different coverage levels using nested downsampling. Top: B cell fraction calculated without IGH haplotype correction. Middle: B cell fraction calculated with germline IGH haplotype correction. Bottom: B cell fraction calculated with both germline and somatic IGH haplotype correction. Throughout, blue lines represent the line of best fit, and gray regions indicate the 95% confidence interval. The P values for Pearson’s R were derived from a two-tailed t-distribution using the correlation coefficient and sample size.
