Extended Data Fig. 8: Identification of factors accounting for loss of type 2 and SFTPA2⁺ features in prostate cancer.

a, Spatial transcriptomic slices of patient 2 colored by CNV Leiden clusters. Loss of PTEN was detected in tumor region inside the red dashed line. b, Heatmap grouped by CNV Leiden clusters showing CNA in each spot. c, Spatial transcriptomic slices colored by gene signatures calculated by AddModuleScore and expression level of PTEN (middle) in each spot. d, Chromosomal location of SFTPA2 and PTEN on chr 10 of human. e, Boxplot showing the type 2 and type 1 signature in each ETS⁻ sample of TCGA-PRAD grouped by Gleason score (top) and pathological T stage (bottom). Gleason 6: n = 30; 7: n = 67, ≥8: n = 38; pT stage T2: n = 56, T3: n = 75, T4: n = 2. Adjusted P values were calculated by two-sided Tukey’s test. The central line represents the median value, box limits indicate the interquartile ranges and the whiskers extend to 1.5× interquartile range. f, Heatmap showing expression levels of selected genes in a CRPC cohort. Expression level of each gene was transformed into row z score before visualization.