Extended Data Fig. 8: Details on FACS gating. | Nature Genetics

Extended Data Fig. 8: Details on FACS gating.

From: Chromothripsis-associated chromosome 21 amplification orchestrates transformation to blast-phase MPN through targetable overexpression of DYRK1A

Extended Data Fig. 8

a. Sorting strategy for mini-bulk RNA, ATAC and 10X single cell RNA-seq experiments: Lineage-CD34+ cells were sorted for subsequent library preparation for data shown in Fig. 3, Fig. 4, Fig. 5g, Extended Data Figs. 3 and 7f. b. Gating strategy for Annexin V/PI apoptosis staining experiments shown in Fig. 7h and Extended Data Fig. 7g. MNCs: Mononuclear cells, FSC-A: Forward Scatter Area, SSC-A: Side Scatter Area, FSC-H: Forward-Scatter Height, 7-AAD: 7- aminoactinomycin D.

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