Extended Data Fig. 3: Molecular barcoding of LM2 cells.
From: Phylogenetic inference reveals clonal heterogeneity in circulating tumor cell clusters
a, Clonetracker XP plasmid map and structure of the barcode cassette obtained from SnapGene software v7.0.0 (from Dotmatics; available at snapgene.com). b, Graphical representation of the experimental design with target multiplicity of infection. c, Left, plot showing the simulated mean proportion of uniquely barcoded cells within sampled cell pools of varying complexities for in vivo engraftment as a function of the number of initially transduced cells (10,000× resampling; Supplementary Note 2). Error bars, s.d. Vertical red line at 151,800 represents the actual number of transduced cells in the conducted experiment as determined via FACS. Right, table depicting the mean and s.d. of the proportion of unique barcodes in sampled pools of 102, 103, 104 and 5 × 104 cells, 72 h after successful transduction of 151,800 cells (10,000× resampling). Panel b is created with BioRender.com. MOI, multiplicity of infection.
