Extended Data Fig. 4: Quality filtering of barcoded CTC clusters.
From: Phylogenetic inference reveals clonal heterogeneity in circulating tumor cell clusters
Plot showing the ratio of the smallest number of barcodes accumulating 90% of total aligned reads over the number of cells per cluster (y axis, log2 scale), for CTC clusters included (number of barcodes/number of cells > 1, filter status = pass, n = 426) and removed (number of barcodes/number of cells > 1, filter status = out, n = 94) from the analysis. The horizontal line at y = 0 illustrates the cut-off for filtering. For comparison, the smallest number of barcodes accumulating 90% of aligned reads in negative control samples (n = 6), containing only lysis buffer without cells, is depicted.
