Extended Data Fig. 3: Effect of CDS2 loss on PA and PIPn synthesis.
The effect of CDS2 loss following treatment with 0.1 µg ml−1 of doxycycline for 7 days or the equivalent volume of DMSO (vehicle) on the synthesis of the major acyl chain species of PA and PI, PIP and PIP2 in MP41 and OMM2.5 cells. Values were calibrated to 34:1 PC and normalized to the mean DMSO values obtained at days 3, 5 and 7. Data are represented as mean ± SEM (n = 3) from three independent MP41 clones and one OMM2.5 clone across 3 independent experiments. Significance determined from analysis of viable and combined apoptotic/dead cell populations is shown. A t-test (two-sided) was used and exact P values < 0.05 are shown. Dox, doxycycline; DMSO, dimethyl sulfoxide; PA, phosphatidic acid; PI, phosphatidylinositol; PIP, phosphatidylinositol monophosphate; PIP2, phosphatidylinositol bisphosphate; PIPn, phosphoinositide.
