Extended Data Fig. 10: MusD-Dac1J adopts the expression of genes surrounding its insertion. | Nature Genetics

Extended Data Fig. 10: MusD-Dac1J adopts the expression of genes surrounding its insertion.

From: Enhancer adoption by an LTR retrotransposon generates viral-like particles, causing developmental limb phenotypes

Extended Data Fig. 10

a, b Schematic representation of the Dac1J -5LTR-LacZ knock-in at the Shh (a) and Sox9 (b) locus. c, whole mount in situ hybridization of Shh from E10.5 wild-type embryo. Scale bar 500um. n = 3 biological replicates were confirmed. d, beta-galactosidase staining on E10.0 Dac1J-5LTR-LacZ (Shh knock-in) showing whole embryos. Scale bars 500um, n = 3/4 embryos show similar staining. e, whole mount in situ hybridization of Sox9 from E12.5 wild-type embryo. Scale bar 500um. n = 3 biological replicates were confirmed. f, beta-galactosidase staining on E12.5 Dac1J-5LTR-LacZ (Sox9 knock-in) showing whole embryos. Scale bars 500um, n = 2/6 embryos show similar staining. g, anti-GAG-MusD whole-mount immuno-fluorescence on E11.5 Dac1J-mutPBS KI/KI (Lbx1 knock-in) showing forelimb staining. Scale bars 100um. At least n = 3 embryos were tested. h, Lysotracker staining of an E12.5 Dac1J KI/KI (Lbx1 knock-in) forelimb. Expected AER staining for that stage is observed but no muscle cell progenitor staining is detected. n = 3 embryos were analysed. Scale bars 100 um.

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