Fig. 6: MusD-Dac1J adopts the muscle progenitor expression when inserted in Lbx1 TAD. | Nature Genetics

Fig. 6: MusD-Dac1J adopts the muscle progenitor expression when inserted in Lbx1 TAD.

From: Enhancer adoption by an LTR retrotransposon generates viral-like particles, causing developmental limb phenotypes

Fig. 6

a, Representation of the three knock-in lines engineered in the Lbx1 TAD. Purple band shows the location of the Lys-tRNA primer binding site, as wild-type and mutated (*). b, In situ hybridization for Lbx1 in E10.5 wild-type showing whole embryos and forelimb. Scale bars, 500 µm. At least n = 3 biological replicates were confirmed. c, β-galactosidase staining on E10.5 Dac1J-5LTR-LacZ (Lbx1 knock-in) showing whole embryos and forelimb. Scale bars, 500 µm. n = 10 out of 10 embryos show similar staining. d, Anti-Gag-MusD whole-mount immunofluorescence on E11.5 Dac1J KI/KI (Lbx1 knock-in) showing whole embryo (left) and forelimb (right). At least n = 3 biological replicates were confirmed.

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