Extended Data Fig. 6: The construction and validation of HuHSC-NCG mice, related to Fig. 5. | Nature Genetics

Extended Data Fig. 6: The construction and validation of HuHSC-NCG mice, related to Fig. 5.

From: Single-cell and spatial transcriptomics reveal mechanisms of radioresistance and immune escape in recurrent nasopharyngeal carcinoma

Extended Data Fig. 6

(a) Schematic illustration of the construction process of the humanized mouse model. (b) After establishing the humanized mouse model, the percentage of mCD45 cells, hCD45 cells, hCD19 cells, and hCD3 cells among PBMC was detected in 18 of the mouse models by flow cytometry. (c) Western blotting for the expression of Col4a1 in MCAM+ CAFs and Col4a1 knockout MCAM+ CAFs. (d) Scatter plot showing the correlations between the expression level of COL4A1 and the ratio of CD8+ T, and CD4+ T cells in spatial data. Two-sided Spearman’s correlation test. (e) C666 cells (1×106) were combined with MCAM+ mCAFs with or without COL4A1 KO and injected subcutaneously into HuHSC-NCG mice followed by irradiation (n = 5 per group). Box plot showing the ratio of CD8+T cells and subgroups of CD8+ T cells in the tumor mice using flow cytometry. Two-sided t-test.

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