Extended Data Fig. 9: SIRPα+ myeloid cells reside near CD47+ malignant cells to inhibit antigen-presentation in rNPC, related to Fig. 8. | Nature Genetics

Extended Data Fig. 9: SIRPα+ myeloid cells reside near CD47+ malignant cells to inhibit antigen-presentation in rNPC, related to Fig. 8.

From: Single-cell and spatial transcriptomics reveal mechanisms of radioresistance and immune escape in recurrent nasopharyngeal carcinoma

Extended Data Fig. 9: SIRPα+ myeloid cells reside near CD47+ malignant cells to inhibit antigen-presentation in rNPC, related to Fig. 8.

(a) UMAP plot showing myeloid cells, color-coded according to PT (blue) and RT (red). (b) t-SNE plot showing the expression of marker genes for the identification of the indicated cell subtypes in Fig. 8a. (c) Scatterplots showing M1 and M2 scores (left panel), as well as pro- and anti-inflammatory scores (right panel) for each color-coded macrophage subtypes. (d) Colocalization scores of TME cells to malignant cells in PT (left) and RT (right). (e) Differences in colocalization scores of TME cells to malignant cells between PT and RT samples. (f) Bar chart showing the number of significant LR interactions from malignant to myeloid subtypes in PT (blue) and RT (red) samples. (g) Scatterplot showing the association between the expression of SIRPA in myeloid subtypes and the distance of these subclusters to CD47+ malignant cells in PT samples, inferred from spatial transcriptomics (ST). Two-sided Spearman’s correlation test.

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