Fig. 6: Partitioned PRS and GWAS enrichment results for four key cardiometabolic traits, stratified by cell-type-level DMRs and compartments.
From: Single-cell DNA methylome and 3D genome atlas of human subcutaneous adipose tissue
a,b, Lollipop plots depict the incremental variance explained of each cell-type-level PRS for abdominal obesity (using WHRadjBMI as a proxy) from the (a) A and B compartments and (b) hypomethylated and hypermethylated regions. Each lollipop represents a WHRadjBMI PRS, in which dot size corresponds to the incremental variance explained of the PRS. Significant enrichment of incremental variance explained was determined empirically by comparing to n = 10,000 permutated PRSs (see Methods). The gray vertical dashed line indicates the significance cutoff based on unadjusted one-tailed permutation P values (that is, Pperm10,000 < 0.05). Bars and lollipops corresponding to cell types with significantly enriched PRSs are colored by cell type, whereas those without are outlined in gray without a filling. c, Summary table visualizing the overall PRS and GWAS enrichment results across four key cardiometabolic traits (that is, WHRadjBMI, MASLD, BMI and CRP), stratified by cell-type-level hypomethylated regions and A compartments. Orange indicates nominal significance (P < 0.05) on the one-tailed permutation P values for PRSs and the one-tailed hypergeometric P values for GWAS enrichment. The bolded black border highlights the following consistently significant cell-type-cardiometabolic trait pair: adipocyte–WHRadjBMI.
