Fig. 4: BRG1 dosage sensitivity of enhancer accessibility is associated with enhancer activity.

a, Histograms of delta values for BRG1-dependent regulatory elements, including all elements, promoters, enhancers and insulators. The red line indicates delta = 0. b, Heatmap showing BRG1-dependent enhancer regulatory elements clustered into five groups based on delta values, ranging from buffered to linear response regions. c, Representative genome browser views showing linear and buffered responses to BRG1 dosage. Red dots represent CUT&RUN signals under different dTAG13 concentrations, and blue dots represent ATAC-seq signals (top) under different dTAG13 concentrations. Genome browser view showing BRG1 binding at regions with linear and buffered responses (bottom). The linear and buffered models are illustrated in the diagram. d, Enrichment analysis of different element types per group, with enrichment calculated against all consensus regions. Dot size represents −log₁₀(P value), and the color scale corresponds to the effect size measured as an odds ratio. Two-sided Fisher’s exact test was used to compare enrichment to background regions. Obs_Exp represents observed divided by expected frequencies. e, Heatmap showing relative enrichment of chromatin marks and TF binding across different groups of ATAC-seq peaks. f, Number of SEs in each group. g, Genome browser view showing ATAC-seq signals at BRG1-bound Capns1 SE at different dTAG13 concentrations. The linear (black) and buffered (green) models are illustrated in the diagram.