Extended Data Fig. 7: Mouse endometrial epithelial cell purification.

(a) Schematic illustrating the experimental design for the collection of uterine horns, isolation of endometrial epithelial cells and downstream analyses. Uterine horns were used for formalin fixation and paraffin embedding followed by immunohistochemistry (left) and for RNA sequencing (RNA-seq, right) from mice as indicated. (b) Relative quantitative reverse transcription PCR (qRT-PCR) of Epcam and Vim mRNA expression in mouse endometrial cell populations isolated from a tamoxifen-treated mouse (n = 1) to confirm the purity of the isolated cell fractions. Gapdh was used as reference. Each symbol represents a technical replicate; center line depicts mean; error bars represent SEM.