Fig. 4: Global interaction and chromatin change of the X chromosome during meiotic silencing. | Nature Genetics

Fig. 4: Global interaction and chromatin change of the X chromosome during meiotic silencing.

From: Chromatin dynamics of a large-sized genome provides insights into polyphenism and X0 dosage compensation of locusts

Fig. 4: Global interaction and chromatin change of the X chromosome during meiotic silencing.The alternative text for this image may have been generated using AI.

a, Correlation of gene expression in brains, leg muscles and gonads. Error bands, 95% confidence interval of the linear model fit. P values < 2.2 × 10−16, Pearson correlation test. b, Expression ratios between X-linked and autosomal genes in gonads under different minimum TPM cutoffs. Error bands, 95% confidence interval around the fitted smoothing curve. c, Pearson correlations of Hi-C interaction frequency matrices of chr. 1 and chr. X at 500-kb resolution. The intensity of each pixel represents the normalized frequency of interaction between a pair of genomic loci. Arrowheads indicate parallel lines along the main diagonal of the interaction map. d, A log–log plot of interaction frequency decay with distance in testes. We assumed independence between the X chromosome and the autosomes. Normalized Hi-C data binned at 25-kb scale were converted to interaction frequencies. The x axis shows the log10 genomic distance, and the y axis shows the median log10 Hi-C interaction frequency for any given genomic distance. e, Relative enrichments of trans contacts by calculating the ratio of the number of trans- over cis-mapping read pairs for each chromosome. f, Condensation of the X chromosome in a meiotic cell of spermatocytes in testes. A series of 24 DNA probes (the single-copy X-linked gene Pex5) was selected randomly. Nuclei were stained with Hoechst 33342; n = 2 independent biological replicates. The position of the X chromosome is indicated by arrowheads. g, Read abundances of H4K16ac and H4K20me3 are shown in 1-Mb bin units. P = 0.0000, *P < 0.05. h, The distribution and abundance of H4K16ac in testicular follicles of adult locusts. Nuclei were stained with Hoechst 33342 staining; n = 2 independent biological replicates. i, Association of Hi-C interaction frequency with gene expression and H4K16ac signals in chr. 1 and chr. X. The A and B compartments, determined by the first principal component (PC1), reflected the transcription and H4K16ac state on a linear genomic scale.

Back to article page