Fig. 6: Interactions between TFs, the cell cycle and differentiation (adipogenesis).

a, UMAP plot of the TF atlas colored by adjusted cell cycle phase (Supplementary Note 7). b, Bar plot showing the fraction of cells in the adjusted phase for each TF. The total number of cells is indicated in brackets. A Fisher’s exact test was performed between confluent control cells (Ctr.conf) and each TF. In addition to Ctr.conf, only TFs and the non-confluent control cells (Ctr.non.conf) that tested significantly (FDR-adjusted P < 0.05) are visualized here. The top three TFs and controls are highlighted in red. c, Density plots showing the distributions of S and G2/M scores of TF cells (T, E2F2 or MYCN in red) compared to confluent control cells (Ctr.conf in teal). d, Bar plots showing the fraction of cells in each adjusted cell cycle phase across binned doses of T, E2f2 or Mycn. e, Heatmaps showing the transcriptomic adiposcore and the mean expression level of p21 in CEBPA, PPARG and MYCN cells, which are binned according to their adjusted cell cycle phase and TF dose. Bins with less than three cells were excluded (white square). f, Fluorescence images showing the viability of control, CEBPA and MYCN cells, indicated by PI staining in red (Supplementary Note 12). Nuclei were stained with Hoechst in blue. Representative images of two independent experiments. Scale bar = 200 μm. See Supplementary Table 5 and Methods for statistics and exact P values (Extended Data Fig. 8). PI, propidium iodide.