Extended Data Fig. 1: Data quality control and dose validation, related to Fig. 1. | Nature Genetics

Extended Data Fig. 1: Data quality control and dose validation, related to Fig. 1.

From: Dissecting the impact of transcription factor dose on cell reprogramming heterogeneity using scTF-seq

Extended Data Fig. 1

a, Violin plots presenting the number of detected genes and counts, the percentage of mitochondrial and ribosomal gene expression of each cell across batches after quality control. b, Ordered cumulative frequency (cum. freq) of the number of cells assigned to each TF in each batch. The dashed line shows the cumulative ordered frequency of a uniform distribution as comparison. c, Left: representative fluorescence images showing the RNA transcripts of WPRE (proxy for TF dose) probed by RNAscope and mCherry (protein) in mCherry and control cells. Red and purple outlines indicate the cell boundary and expanded cell boundary, respectively. Representative images of two independent experiments. Scale bar = 100 μm. Right: single-cell quantification showing the correlation between the log-normalized mean fluorescence intensity of mCherry versus WPRE mRNA in mCherry and control cells. Fitted model: LOESS.

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