Extended Data Fig. 2: A wide dose range generated by scTF-seq, related to Fig. 1.

a, Volcano plots showing differential gene expression between control (mCherry) and FOS cells in our study (batch 9) and ref. ¹⁶. The q-value is the adjusted P value generated by the t test followed by FDR correction. b, Dose-response curves (top) and exemplary UMAP plots (bottom) for the expression of the top six shared up- and down-regulated genes in response to FOS overexpression in the scTF-seq dataset. These genes were selected by first filtering on significant (FDR <5%) differential expression in both the scTF-seq and Joung et al.¹⁶ datasets, and subsequently selecting the top six differentially expressed genes in ref. ¹⁶ dataset. c, UMAPs for the expressions of the orthologous genes from b on ref. ¹⁶ dataset. d, Standard boxplots (Methods) showing the distribution of fold changes when filtering the scTF-seq cells according to several maximal dose cutoffs (red) compared to the distribution of differential expression from ref. ¹⁶. e, UMAP plot of the TF atlas colored by cell cycle phase assigned with a default Seurat cutoff of 0 for cell cycle scores.