Fig. 2: Cellular landscape of early human cardiogenesis.
From: Spatiotemporal gene expression and cellular dynamics of the developing human heart
a, Overview of Chromium single-cell dataset generation and spatially informed annotation of cardiac cell states, with donor numbers by PCW shown in blue circles. DGE, differential gene expression. b, UMAP representing 31 coarse-grained cardiac single-cell clusters, corresponding to 11 main cell types, including CMs, ECs and non-mural MC–FBs (dashed lines). c, Enrichment of canonical cell type markers across coarse-grained single-cell clusters. Dashed rectangles highlight consensus markers of CMs, ECs and FBs. d, Spatiotemporal mapping of selected coarse-grained clusters in 6, 8, 10 and 12 PCW heart sections. e, Spatially aware annotation of coarse-grained CM, EC and MC–FB clusters. f, Temporal changes in coarse-grained cluster proportions in the CM, EC and MC–FB subsets across four developmental age groups (5.5–6, 7–8, 9–11 and 12–14 PCW). ▲2×, ▲▲3×, ▲▲▲4× increase; ▼2×, ▼▼3×, ▼▼▼4× decrease between the 5.5–6 and 12–14 PCW age groups.
