Extended Data Fig. 8: PTGES3 potentially interacts with histone acetyltransferase KAT2A.
a, LNCaPi cells were infected with sgCTRL or sgPTGES3 and selected with puromycin. KAT2A, P300, FOXA1, PTGES3, and GAPDH protein levels were detected by western blotting. b, IP experiments were performed with LNCaP cell lysate using an IgG or PTGES3 antibody. KAT2A, P300, FOXA1, and PTGES3 protein levels were detected by western blotting. c, LNCaP cells containing ARE-luciferase reporter were transfected with pCDH-PTGES3. Cells were treated with siRNA targeting control or KAT2A in the condition of 1 nM DHT. Luciferase activities over Renilla were normalized with control (n = 3 as biological replicates; Mean ± SD). Two sided t-test was used to determine statistical significance (***P < 0.001). d. LNCaP cells were fixed sequentially by EGS and formaldehyde. Dual cross-linking ChIP experiments were performed using indicated antibodies. Precipitated DNA was used as a template to amplify the indicated genomic regions by real-time PCR (n = 3 as biological replicates; Mean ± SD). LKB1 serves as a positive Dual X ChIP control which indirectly binds to the P21 promoter (non-ARE) by interacting with P53. The P21 promoter levels in LKB1 group were compared to the IgG group, two sided t-test was used to determine statistical significance (***P < 0.001).
