Extended Data Fig. 1: Intratumoural pluripotent haematopoietic stem progenitor cells possess antigen-presentation machinery.
From: Childhood brain tumors instruct cranial hematopoiesis and immunotolerance
a, Messenger ribonucleic acid (mRNA) transcript expression of genes encoding antigen presentation molecules and machinery in fluorescent activated cell-sorting (FACS)-isolated LSK+ cells, isolated from EPZFTA-RELA tumours, compared to LSK− cells (n = 7, 10 pooled mice per replicate). b, Experimental design pertaining to Figures b–e. c, Peripheral blood engraftment of MHC-II Lin- cells transplanted from tumors over 16 weeks (n = 6, 8 pooled mice per replicate) d, Quantification of bone marrow engraftment of MHC-II+ and MHC-II- tumor-derived Lin- cells (n = 6/group, mean±s.e.m., unpaired two-tailed t-test). e, Bone marrow engraftment of MHC-II+ Lin- cells in different cell types after primary and secondary transplantation; multipotent progenitor (MPP)3/4, MPP2, MPP1, haematopoietic stem cell (HSC), granulocyte-monocyte precursor (GMP) pre-megakaryocyte/erythrocyte (PreMegE), pre- granulocyte monocyte (preGM), pre-colony-forming unit-erythroid cell (CFU-E), CFU-E, LSK-, CD8 T cell (TC), CD4 TC, neutrophil (neutro.), monocyte (mono.), macrophage (macro.) eosinophil (Eos), B cell (BC) and dendritic cell (DC). Illustrations in b created using BioRender.com.
