Extended Data Fig. 2: Cerebrospinal fluid (CSF) interacts with the skull bone marrow in EPZFTA-RELA-bearing mice.
From: Childhood brain tumors instruct cranial hematopoiesis and immunotolerance
a, Volcano-plot of differential expression analysis of CSF proteins in EPZFTA-RELA-bearing relative to control bearing mice (n = 6/group), using a limma-based moderated t-test. b, t-distributed stochastic neighbour embedding (t-SNE) visualizations of scRNA-seq from dorsal skull and tibial bone marrow from 2-month-old mice coloured by cell type; B cells, T cells, natural killer (NK) cells, haematopoietic stem progenitor cells (HSPCs), innate lymphoid cells (ILCs), neutrophils, monocytes, mast cels and dendritic cells (DCs). c, Chord plot detailing between CSF ligands identified by tandem mass tag (TMT) Liquid chromatography–mass spectrometry (LC–MS) and receptors on skull bone marrow HSPCs, B cells, monocytes and neutrophils identified by scRNA-seq. d, Gene Ontology (GO) pathway analysis on receptor genes with at least one CSF ligand in HSPCs, neutrophils and monocytes.
