Extended Data Fig. 3: Clonal expansion of T cells in the dura and skull bone marrow of EPZFTA-RELA-bearing mice.
From: Childhood brain tumors instruct cranial hematopoiesis and immunotolerance
a, uniform manifold approximation and projection (UMAP) visualisation of single-cell RNAseq of T cell subsets from extravascular CD45+ cells isolated from the skull and tibia bone marrow, dura, deep cervical lymph nodes (dcLN) and brain/tumour from EPZFTA-RELA-bearing and control bearing mice, coloured by cell type; CD8 tissue effector memory (CD8 TEM) CCR9+, CD4 tissue resident memory (CD4 TRM), CD8 TEM CCL5+, regulatory T cell (Treg), CD8 TEM, douple-positive (DP) T cell, CD8 TEM (NR4A1+), CD4 tissue central memory (TCM), gamma-delta (gd) T cells, CD8 TEM CD266+). b, Stacked barplot of T cell populations across tissues in EPZFTA-RELA-bearing and control bearing mice. c, UMAP of single-cell RNAseq of T cell subsets split by tissue and genotype. d, variable–diversity–joining rearrangement (VDJ) T cell receptor (TCR) clone network across tissues in EPZFTA-RELA-bearing mice. e, circle plot of shared T cell receptor clones between tissues in EPZFTA-RELA-bearing mice. f, circle plot of shared T cell receptor clones between cell types in dura of EPZFTA-RELA-bearing mice. g–i, Bar plot of expanded clones in top 4 expanded T cell populations in each tissue.
