Extended Data Fig. 3: Single-cell expression analysis of candidate genes in human and mouse cells in intestinal tissues. | Nature Genetics

Extended Data Fig. 3: Single-cell expression analysis of candidate genes in human and mouse cells in intestinal tissues.

From: Genome-wide association analyses highlight the role of the intestinal molecular environment in human gut microbiota variation

Extended Data Fig. 3

a,b, Single-cell expression analysis of candidate genes in human duodenal, ileal, and colonic epithelial (a) and immune (b) cells from donors. Heatmaps of mean gene expression were generated from the different intestinal epithelial and immune cell clusters in the dataset from Hickey et al.22. T-cells data were absent for duodenum. c, Single-cell expression in mouse enteroendocrine cells (EECs) from different regions of the gut24. Mean gene expression in different EEC clusters per gastrointestinal (GI) region. EECs were purified by flow cytometry from NeuroD1-Cre/YFP mice, and analyzed by 10× single-cell RNA sequencing. Cells were clustered by k-means and annotated according to their expression of gut hormone genes: D (somatostatin), G (gastrin), I (cholecystokinin), K (glucose-dependent insulinotropic polypeptide), L (glucagon-like peptide 1 and peptide YY), Insl5 (insulin-like peptide 5), N (neurotensin), X (ghrelin), EC (enterochromaffin cells expressing Tph1 as a marker for serotonin biosynthesis), ECL (enterochromaffin-like cells expressing histidine decarboxylase as a marker for histamine biosynthesis). USI, upper small intestine; LSI, lower small intestine; LI, large intestine.

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