Extended Data Fig. 4: Single-cell expression in enteroendocrine cells from duodenal and ileal human-derived organoids. | Nature Genetics

Extended Data Fig. 4: Single-cell expression in enteroendocrine cells from duodenal and ileal human-derived organoids.

From: Genome-wide association analyses highlight the role of the intestinal molecular environment in human gut microbiota variation

Extended Data Fig. 4

a,b, Feature maps represent gene expression in human enteroendocrine cells (EEC) clusters from the duodenum (a) and ileum (b)23. EECs were labeled by inserting a yellow fluorescent protein downstream of the Chromogranin A promoter in organoids, by CRISPR-Cas9. Fluorescent cells were purified by flow cytometry and analyzed by 10× single-cell RNA sequencing. Cells were clustered by k-means and annotated according to their expression of gut hormone genes: D (somatostatin), I (cholecystokinin), K (glucose-dependent insulinotropic polypeptide), L (glucagon-like peptide 1 and peptide YY), M/X (motilin and ghrelin), EC (enterochromaffin cells expressing TPH1 as a marker for serotonin biosynthesis).

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