Extended Data Fig. 3: Generating and validating E7 TCR T cells. | Nature Genetics

Extended Data Fig. 3: Generating and validating E7 TCR T cells.

From: High-content CRISPR activation screens identify synthetically lethal RNA-based mechanisms to sensitize cancer cells to targeted T cell cytotoxicity

Extended Data Fig. 3: Generating and validating E7 TCR T cells.

(a) Western blot of the HPV16 E7 protein in CaSki cells. 293 T and HeLa cells serve as negative controls. This readout was obtained once to complement E7 presentation by CaSki cells, confirmed repeatedly via E7-TCR-dependent cytotoxicity. (b) Flow cytometry analysis of HLA-A2 in CaSki cells. 293 T cells serve as positive controls. (c) E7 TCR construct. (d) Representative FACS plots of T cells transduced to express the E7 TCR construct and stained with an mTRBC antibody to isolate E7 TCR T cells. (e) Percentage of CaSki cancer cells killed (mean ± SD, n = 3 technical replicated per group, y axis) after 48 h in coculture with parental or E7 TCR T cells at 5:1 E:T (left) or with E7 TCR T cells at varying E:T ratios (x axis; right). Left: ****P < 0.001, unpaired, two-tailed t-test. Right: ****P < 0.001, ordinary one-way ANOVA, Dunnett’s multiple comparisons test. Illustration in c created in BioRender; Jerby Lab https://biorender.com/qdelxmb (2026).

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