Extended Data Fig. 4: Perturb-seq screen confirms CRISPRa on-target gene activation (part 1). | Nature Genetics

Extended Data Fig. 4: Perturb-seq screen confirms CRISPRa on-target gene activation (part 1).

From: High-content CRISPR activation screens identify synthetically lethal RNA-based mechanisms to sensitize cancer cells to targeted T cell cytotoxicity

Extended Data Fig. 4: Perturb-seq screen confirms CRISPRa on-target gene activation (part 1).

(a) Expression level (color bar, shown as centered and scaled log1p-transformed average tp10k values) and percentage of cells expressing each of the target genes (rows) in cells stratified based on the sgRNA target (columns). (b) Expression levels as in (a) and percentage of cells expressing each of the target genes (columns) in cells stratified based on the individual sgRNA (rows), shown for a subset of the sgRNAs. In red are sgRNAs that do not show a statistically significant overexpression of the target gene (BH FDR > 0.05, edgeR test).

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