Extended Data Fig. 6: Perturb-seq coculture GA signature validation in monoculture.

GA signatures derived separately from monoculture (black and pink) or coculture (blue and yellow) were applied to classify cells as subject to the pertaining gene activation, when considering all cells in the Perturb-seq monoculture dataset, or only control (NTC) and cells with the pertaining gene activation based on sgRNA detection in monoculture. Only GA signatures with at least 5 genes were used as predictors. Mono (monoculture), co (coculture).