The Single Cell Notebooks provide multilingual, open-access training materials for single-cell and spatial transcriptomics analysis through reusable notebooks. They have evolved into a community-driven platform for education and capacity building, lowering language and computational barriers to support equitable participation in omics research.
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References
Picelli, S. RNA Biol. 14, 637–650 (2017).
Maracaja-Coutinho, V. & Severino, P. in Transcriptomics in Health and Disease (ed. Passos, G. A.) 127–141 (Springer, 2022).
Wang, S. et al. Int. J. Mol. Sci. 24, 2943 (2023).
Atta, L. & Fan, J. Nat. Commun. 12, 5283 (2021).
Lähnemann, D. et al. Genome Biol. 21, 31 (2020).
Liu, X. et al. J. Hematol. Oncol. 17, 72 (2024).
Possik, P. A. et al. Cell 188, 5790–5796 (2025).
Maracaja-Coutinho, V. & Nakaya, H. I. Nat. Rev. Mol. Cell Biol. 27, 1–3 (2026).
Didier, J. et al. Endocr. Relat. Cancer 32, e250024 (2025).
Hemberg, M. et al. Genome Biol. 26, 358 (2025).
Clough, E. et al. Nucleic Acids Res. 52, D138–D144 (2024).
CZI Cell Science Program et al. Nucleic Acids Res. 53, D886–D900 (2025).
George, N. et al. Nucleic Acids Res. 52, D107–D114 (2024).
Armingol, E., Baghdassarian, H. M. & Lewis, N. E. Nat. Rev. Genet. 25, 381–400 (2024).
Acknowledgements
This work was supported by the Agencia Nacional de Investigación y Desarrollo (ANID, Chile): FONDECYT (1211731 to V.M.C. and 3250452 to D.P.S.), FONDAP (15130011 and 1523A0008 to V.M.C.), Anillo (ATE220016 to V.M.C.) and BECAS CHILE (2018–72190270 to E.A.); by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil), through the PrInt program (to V.M.C.); by the Chan Zuckerberg Initiative (CZI, USA) (to A.M.E., J.R., P.S., M.B., Y.C.A., B.S.C. and V.M.C.); by the Gates Foundation (to A.K.S.); by the European Union through a Marie Skłodowska-Curie Actions Postdoctoral Fellowship (grant agreement no. 101208051 to E.A.); and by Pew Latin American Fellowship Program in Biomedical Sciences (to S.T.). We are also grateful to the Wellcome Connecting Sciences for the continuous support in several practical workshops and symposiums organized over the years in the region.
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Rojas-Hidalgo, A., Arias-Carrasco, R., Silva, J.K. et al. The Single Cell Notebooks for inclusive and accessible training in single-cell and spatial omics. Nat Genet (2026). https://doi.org/10.1038/s41588-026-02584-0
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DOI: https://doi.org/10.1038/s41588-026-02584-0