Supplementary Figure 3: RNF2 inhibits IFN-γ-induced ISG transcription
From: Nuclear RNF2 inhibits interferon function by promoting K33-linked STAT1 disassociation from DNA
(a) Q-PCR analysis of mRNA expression of Irf1, Irf8 and Fgl2 in peritoneal macrophages from Rnf2 fl/fl and Rnf2 fl/fl Lyz2-cre mice stimulated with mouse IFN-γ (500 pg/ml) for 6 h. (b) Dual luciferase reporter assay of GAS activity in L929 cells co-transfected with GAS-luciferase reporter vector and RNF2-expressing vector, stimulated with mouse IFN-γ (1 ng/ml) for 12 h. Data are representative of three independent experiments with n = 3 (a), n = 4 (b) technical replicates, each symbol represents an individual technical replicate (a, b) (shown as mean and SEM in a, b), * P < 0.05, two-tailed unpaired Student’s t-test
