Supplementary Figure 4: RNF2 inhibits type I interferon antiviral responses in vivo

(a) Q-PCR analysis of EMCV replicates in the heart, brain, and pancreas of Rnf2 ^fl/fl and Rnf2 ^fl/fl Lyz2-cre mice of 8 weeks age infected with EMCV (1 × 10⁵ PFU per gram body weight) by intraperitoneal injection for 48 h. (c) Immunoblot analysis of protein levels of IFIT1 and ISG15 in the heart and brain from mice described in (b). (d) Q-PCR analysis of mRNA expression of Ifit1, Oas2, and Mx1 in the heart, brain, and pancreas from mice described in (b). (e) Hematoxylin-and eosin staining of heart sections from mice described in (b). Scale bar, 20 μm. (f) Q-PCR analysis of IAV replicates and mRNA expression of Ifit1, Oas2, and Mx1 in the lung of Rnf2 ^fl/fl and Rnf2 ^fl/fl Lyz2-cre mice of 8 weeks age infected with IAV (100 PFU) by intranasal delivery for 24 h. (g) Immunoblot analysis of protein levels of IFIT1 and ISG15 in the lung from mice described in (f). (h) Hematoxylin-and eosin staining of lung sections from mice described in (f). Scale bar, 20 μm. Data are representative of three independent experiments with n = 3 (a, c, e) technical replicates, three independent experiments (b, d, f, g), each symbol represents an individual technical replicate (a, c, e) (shown as mean and SEM in a, c, e), * P < 0.05, ** P < 0.01, *** P < 0.001, two-tailed unpaired Student’s t-test