Supplementary Figure 5: T helper polarization by P1-, P2- and P3-pDCs.
From: Diversification of human plasmacytoid predendritic cells in response to a single stimulus
a ICOS, CD127, L-Selectin and CCR7 surface expression by CD4 T cells after coculture with pDC activated populations b PDC were sorted as CD2– CD5– AXL– and culture 24h with Flu. PDC subpopulations were sorted and coculture with heterologous CD4 naive T cells. T cell expansion was measured after 6 days with P1-, P2- or P3-pDC (P1-T, P2-T and P3-T respectively). Results shown as the median of 3 independent donors. (ANOVA + Tukey’s post-hoc) c T helper cytokines induced by the activated pDC sub populations. Cytokines were measured after 24h polyclonal restimulation of the pDC polarized TH cells. Results include the median for 6 independent donors. (Friedman test + Dunn's post-hoc) d PDC-polarized TH cells were restimulated with PMA and Ionomicine. Intracellular TNF (left) and quantification of TNF producing cells (right). Results shown as the median for 5 donors. ns=non-significant; (ANOVA+ Tukey’s post-hoc). *p < 0.05; **p < 0.01.
