Supplementary Figure 5: MEKK2 deficiency enhances the innate antiviral response

(a, b) qPCR analysis of Ifnb1, Cxcl10 and Isg15 mRNA in wild-type and Map3k2^−/− peritoneal macrophages infected with SeV (a) or transfected with 5′-ppp RNA (b) for the indicated time points. (c) ELISA of IFN-β secretion by wild-type and Map3k2^−/− peritoneal macrophages infected with SeV (left), VSV (MOI, 0.1) (middle) or HSV-1 (MOI, 10) (right) for the indicated time points. (d) Immunoblot analysis of VSV-G in wild-type and Map3k2^−/− peritoneal macrophages infected with VSV (MOI, 0.1) for the indicated time points. VSV specific mRNA is shown in the lower panel. All qPCR results are shown as mean + s.d. (a–c, d lower pannel) of triplicates of at least two independent experiments. *P < 0.05 (two-tailed Student’s t-test.).