Supplementary Figure 4: The chromatin landscape of DKO ABCs shows enrichment for IRF and AP-1–BATF motifs
From: Regulation of age-associated B cells by IRF5 in systemic autoimmunity
(a) Expression (RNAseq) of total ABC-specific peaks associated genes (n = 2,482) from three independent experiments (n = 3) (b) Functionally enriched Gene Ontology (GO) categories of selected genes associated with ABC-specific peaks of ATAC-seq (n = 487). (c) qPCR analysis of the expression of Il6 mRNA in sorted FoB (B220+CD19+CD11c-CD11b-CD23+) cells from WT and DKO female mice and ABC (B220+CD19+CD11c+CD11b+) cells from DKO female mice as indicated. The data were normalized relative to Ppia mRNA expression. Mean of one representative experiment of 2 independent experiments is shown (n = 2 mice/group). SEM of technical replicates is shown. ** P = 0.0052.
