Supplementary Figure 6: IRF5 regulates the IL-21-mediated formation of DKO ABCs

(a) Flow cytometric analysis of B220⁺CD11c⁺CD11b⁺B cells in the spleens of WT, DKO and Cd11c-Cre Irf4^fl/fl DKO female mice (>18 weeks-old). Graphs show frequencies and numbers for individual mice and mean values of 4 independent experiments (n = 4 WT, 5 DKO, 6 Cd11c-Cre Irf4^fl/fl DKO). ns: not significant; * P = 0.0485; ** P = 0.0072. (One-way ANOVA followed by Bonferroni’s multiple comparisons test). (b) qPCR analysis of the expression of Irf5 mRNA in cultures of CD23⁺ B cells purified from WT, DKO and Cd21-Cre Irf5^fl/– DKO female mice (8–10 weeks of age) stimulated with αIgM (5 μg/ml) and αCD40 (5 μg/ml). Data were normalized relative to Ppia mRNA expression. Mean of one representative experiment of 4 independent experiments is shown (n = 4 mice/group). SEM of technical replicates is shown. ns: not significant. (One-way ANOVA followed by Bonferroni’s multiple comparisons test).