Supplementary Figure 6: Absolute quantification of lipids detected in association with CD1c or 3C8 TCR–CD1c using HPLC-TOF-MS
From: T cell autoreactivity directed toward CD1c itself rather than toward carried self lipids
Lipids present in the eluents of the indicated CD1c or CD1c-TCR complex were compared to authentic standards of (a) MAG, (b) PC and (c) SM measured under the same conditions and expressed as area under the curve of the ion chromatogram (count-seconds). These values were used to calculate molar ratios of lipid ligands shown in Figure 5 (right). Data are representative of two independent experiments with similar results.
