Supplementary Figure 8: Sorting of HD1CD4+ and HD1CD4– cells and treatment of MAG rescues staining with a SM-treated CD1c tetramer
From: T cell autoreactivity directed toward CD1c itself rather than toward carried self lipids
(a) HD1CD4– cells and HD1CD4+ cells were stained with CD1c tetramer-APC or not, two weeks after sorting and stimulation with irradiated feeder cells and anti CD3 antibody. (b) CD1c monomers were first treated overnight with SM, or mock treated. Subsequently, ceramide, MAG, or diacylglycerol (DAG) was added and the CD1c-ligand mixtures were incubated overnight for a second time. The tetramerized monomers were used to stain the cell line HD1CD4+ (top), or HD1CD4– (bottom). Data are representative of 2 independent experiments with similar results.
