Supplementary Figure 2: Validation of transcriptional signatures in an independent cohort.

a) 2009/2010 cohort clustered on individuals and transcripts (Pearson’s uncentered with averaged linkage) using 1255 transcript list (from Figure 1). (b) 2009/2010 cohort clustered on individuals and transcripts (Pearson’s uncentered with averaged linkage) using 25 transcript list (from Figure 1). (c) 2009/2010 cohort clustered on individuals and transcripts (Pearson’s uncentered with averaged linkage) using 231 transcript list of severity (from Figure 2, transcripts retained if >2FC between severity 3 and severity 1&2). GO Terms analysis of 3 major branches of the transcripts dendrogram was undertaken and listed next to the heat-map. (d) Using 51 and 112 transcripts lists (from Figure 3) ‘viral response’ and ‘bacterial response’ molecular scores were calculated and plotted for each influenza patient (relative to healthy controls). Cases were coded according to severity of illness, indicated by the colour of the respective dots (severity 1, black; severity 2, blue; severity 3, red).