Supplementary Figure 2: Validation and replication experiments.
From: Integration of multi-omics data and deep phenotyping enables prediction of cytokine responses
(a) HDL cholesterol decreases cytokine production in response to A. fumigatus. Log2 cytokine expression relative to RPMI in PBMCs cultured using LPDP and LPDP+HDL shows a decrease in expression upon stimulation with A. fumigatus. Experiments were performed in 6 healthy individuals. (b) Spearman correlations after age and gender correction between cytokine production and circulating IL-18BP concentration in 500FG (left, n=489) and a subset of individuals from 300-OB filtered on BMI < 28 (right, n=51). (c) Cytokine production of TNF-α and IL-6 by PBMC derived macrophages in response to E. coli and S. aureus is lowered in cells cultured on a medium containing acetate but not for C. albicans. Y-axis shows log2 fold changes in cytokine level compared to the non-acetate condition in macrophages cultured on a medium with acetate and stimulated with C. albicans, S. Aureus and E. coli in 6 healthy Dutch donors. Blue indicates TNFA. Red indicates IL-6.
