Supplementary Figure 1: Phenotypes of tumor-infiltrating lymphocytes.

Supplementary Figure 1. Phenotypes of tumor-infiltrating lymphocytes. (a) Frequency and MFI of TIGIT⁺ cells in CD8⁺ T cells (CD45⁺CD3⁺CD56^–) from peritumoral regions (PT) and intratumoral regions (IT) in patients with CRC (n = 16). (b) Representative flow cytometry plot of TIGIT expression in NK cells from the spleen, liver and lung as described in Fig. 1b. (c) Frequencies of TIGIT⁺ in CD8⁺ T cells in B16-pulmonary metastasis model (n = 4 mice per group). (d) Representative flow cytometry plots of tumor-infiltrating NK cells and TIGIT expression on NK cells in four subcutaneous tumor models. (e) Frequencies of TIGIT⁺ cells in CD8⁺ T cells as described in Fig. 1c (n = 5 mice per group). (f, g) Frequencies of PD-1⁺, or CTLA-4⁺ cells in CD8⁺ or CD4⁺ T cells as described in Fig. 1d (n = 5 mice per group). (h) Frequencies of TIGIT⁺CTLA-4^–, TIGIT⁺CTLA-4⁺, and TIGIT^–CTLA-4⁺ cells in tumor-infiltrating CD4⁺ T cells (n = 5 mice per group). (i) Frequencies of CD226, CD96, LAG3, TIM3, Ly49A, Ly49C/I, NKG2A and NKG2D (n = 16, 16, 15, 23, 5, 5, 5, 5 mice) in TIGIT^– and TIGIT⁺ tumor-infiltrating NK cells in B16-pulmonary metastasis model. (b-i) Data are representative of at least three independent experiments. ns, not significant (P > 0.05), *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; Error bars are mean ± s. e. m. (c, e, f, g); paired two-tailed t-test (a, i); one-way ANOVA followed by Tukey's (c, h) or Dunnett's (e, f, g) multiple comparisons test.