Supplementary Figure 3: Conditional knockout of TIGIT on NK cells prevents the exhaustion of TILs. | Nature Immunology

Supplementary Figure 3: Conditional knockout of TIGIT on NK cells prevents the exhaustion of TILs.

From: Blockade of the checkpoint receptor TIGIT prevents NK cell exhaustion and elicits potent anti-tumor immunity

Supplementary Figure 3

(a) Representative histograms of TIGIT expression on NK and T cells from Tigitfl/fl- Ncr1iCre/+, Tigitfl/fl- Ncr1+/+, Tigit–/– and WT mice. Grey shadow represent isotype staining. The experiment was repeated independently for three times with similar results. (b, c) Tigitfl/fl- Ncr1iCre/+ and Tigitfl/fl- Ncr1+/+ mice were intravenously injected with 1.5 × 105 B16/F10 cells (n = 4). TILs were analyzed 15-d post challenge. (b) Expression of CD226 and CD96 in tumor-infiltrating NK cells. (c) Expression of PD-1 and Tim3 in tumor-infiltrating CD8+ T cells. (b-c) Data are representative of three independent experiments. ns, not significant (P > 0.05), *P < 0.05, **P < 0.01; Error bars are mean ± s. e. m. (b, c); unpaired two-tailed t-test (b, c).

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