Supplementary Figure 3: Accumulation of CD4⁺CD38⁺CCR7^– in whole blood of volunteers with enteric fever

(a) Identification of CD4⁺CD38⁺CCR7^- cells during enteric fever by Multicolour Flow Cytometry in whole blood from a representative volunteer challenged with S. Typhi, and one challenged with S. Paratyphi A. (b) Accumulation of CD38⁺CCR7^- cells after diagnosis in volunteers challenged with S. Typhi and Paratyphi A who developed (Diagnosed) but not in those who did not develop enteric fever (Not Diagnosed). In challenged volunteers not diagnosed blood was collected 14 days after challenge (D14). Friedman Multi comparison Test p = 0.006 (S. Typhi), p < 0.0001 (S. Paratyphi). Dunn’s Multiple Comparison Test ED vs D0, p = 0.0065 (Typhi, n = 9); p = 0.0002 (S. Paratyphi A, n = 9). Box extends from the 25^th to the 75^th percentile and whiskers from the minimum to the maximum value; line indicates median.