Supplementary Figure 4: Gating strategy for live fluorescence barcoding of T cell clones | Nature Immunology

Supplementary Figure 4: Gating strategy for live fluorescence barcoding of T cell clones

From: Clonal analysis of Salmonella-specific effector T cells reveals serovar-specific and cross-reactive T cell responses

Supplementary Figure 4: Gating strategy for live fluorescence barcoding of T cell clonesThe alt text for this image may have been generated using AI.

Shown is an example with four live fluorescent dyes. Cell were labelled with 9 combinations of three distinct dilution of Violet and Far red dyes. Cells with each of the nine cluster were labelled with 6 combinations of 4 distinct dilutions of CFSE and and 3 of CMTMR orange dyes. Dilutions used for adjacent clusters across the 640-670 axis were labelled with alternate dilutions of CFSE and CMTMR Orange (as shown in the overlaid plots) in order to reduce spill-over in case of suboptimal separation between clusters across the channel-dye combination with the lowest stain index.

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