Supplementary Figure 3: IL-17F regulates the colonization of C. XIVa. | Nature Immunology

Supplementary Figure 3: IL-17F regulates the colonization of C. XIVa.

From: Suppression of IL-17F, but not of IL-17A, provides protection against colitis by inducing Treg cells through modification of the intestinal microbiota

Supplementary Figure 3

(a) Three week-old WT and Il17f–/– mice were weaned and co-housed in the same cages for 4 weeks. Then, they were separated into different cages. After the indicated period, fecal microbiota was harvested and the relative content of C. XIVa and Prevotella was determined by qPCR (WT n=7, Il17f−/− n=5). *P=0.0119, **P=0.0057 (unpaired two-tailed Student’s t-test). (b) The expression of indicated genes encoding AMPs in WT, Il17f−/− or Il17a−/− mouse colon was examined by qPCR (in panels from Lcn2 to Defa4, n=4/group; in other panels, WT n=12, Il17f–/– n=8, Il17a–/– n=8). *, #: P<0.05. *P=0.0333, **P=0.007, ***P=0.0005, #P=0.0433 (one-way ANOVA followed by Tukey’s multiple-comparisons test). Data are expressed as means ± SD.

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