Supplementary Figure 4: Stage-specific transcriptional signatures define pDC commitment.
From: Distinct progenitor lineages contribute to the heterogeneity of plasmacytoid dendritic cells
(a-f) RNASeq was performed on sort purified DN, SP, DP and mature BM pDCs as described in Methods. (a,b) Shown are developmental heatmaps, as described in Fig 4c, for transcription factors with expression values log2FC >1.5 (a), or cell-surface markers with expression values log2FC > 3 (b). (c) Shown is Gene-Set Enrichment Analysis (GSEA) performed on Hallmark Signatures comparing DP pre-pDC progenitors with mature pDCs. (d-f) Volcano plots showing pair-wise comparisons of DP pre-pDC against DN, SP and mature pDC transcripts as indicated. Depicted in red are up-regulated transcripts (log2FC > 1), in blue down-regulated transcripts (log2FC < -1). (n=4 independent experiments. For each experiment, all progenitors were obtained from one mouse).
