Supplementary Figure 2: Single molecule blinking statistics.
From: TCRs are randomly distributed on the plasma membrane of resting antigen-experienced T cells
Blinking statistics of labels used for label-density-variation SMLM experiments derived from time traces of recorded localizations. Data were recorded on fixed primary murine CD4+ TEFF cells labeled with antibody concentrations or PS-CFP2 expression levels so low that well-separated single molecule signals could be observed. (a) ton: time a single molecule was detectable in consecutive frames, toff: time a molecule remained in a dark state. (b) Histograms summarizing the blinking data with respect to the total number of observation per molecule, N, ton, toff and the first frame in which a molecule was detected; n = 1,827 for H57-AF647, n = 6,151 for KT3-AF647 and n = 1,409 for PS-CFP2
