Supplementary Figure 3: Splenic sorted CD8+ DCs produce IL-12 in response to 2216PS and VACV70 independent of the STING pathway.
From: PLD3 and PLD4 are single-stranded acid exonucleases that regulate endosomal nucleic-acid sensing
WT and Tmem173–/– sorted CD8+ DCs were plated in duplicates, stimulated with the indicated agents overnight and cytokine release measured. (a) IFN-λ production, measured by ELISA. (b) IL-12p70 production, measured by Luminex assay. (c) Comparison of IL-12p70 production (measured by ELISA) of CD8+ cDC sorted from mice deficient in IFN-γ alone or lacking both IFN-γ and PLD4. Duplicate cultures shown. Experiment performed twice. (d) Sorted splenic CD8–CD11bhi DCs produce IL-12p70 and IL-6 to indicated ligands. DCs plated in triplicate. Experiment performed twice. In all graphs, mean and standard deviation are shown. (c-d) Statistics were determined using a non-paired two-tailed Student’s T test assuming equal variance.
