Supplementary Figure 5: Comparative efficiency of digestion of PLD4 and PLD3 for phosphorothioate (PS) and phosphodiester (PO) linked ODNs.
From: PLD3 and PLD4 are single-stranded acid exonucleases that regulate endosomal nucleic-acid sensing
The indicated concentrations of mouse PLD3 or PLD4 were used to digest the indicated ODNs (2.5 μM) in 50 mM MEF pH 5.0, 125 mM NaCl at 37oC for 2 hours, electrophoresed on 20% polyacrylamide/TBE/urea gels and stained with Sybr gold. CpG-ODNs were provided in excess, 2.5 μM. This experiment was performed four times with similar results.
