Supplementary Figure 7: Analysis of Pld3–/–Pld4–/– animals and irradiated recipients of bone marrow from Pld3–/–Pld4–/– animals.
From: PLD3 and PLD4 are single-stranded acid exonucleases that regulate endosomal nucleic-acid sensing
(a) Weanling expected and observed genotype results from F2 crosses of either Pld3–/–Pld4+/– (left) or Pld3+/–Pld4–/– (right) parental breeding schemes. A Pearson’s Chi Square test was performed. (b) A 16 day-old litter from Pld3–/–Pld4+/– x Pld3+/–Pld4–/– breeding with genotype of pups shown. (c) Macroscopic image of liver from Pld3–/–Pld4–/– animal depicted in (b). (d) MHCII and CD86 expression levels of peritoneal macrophages isolated from bone marrow chimeric mice receiving bone marrow from either Pld3–/–Pld4+/– (red), Pld3+/–Pld4–/– (blue), or Pld3–/–Pld4–/– (green) donors. MHCII and CD86 expression levels of peritoneal macrophages from a control C57BL/6 animal (orange) are shown for comparison. Experiment performed once. Blood monocyte levels (e, i), serum cytokine IFN-γ (g), IFN-α (h), IL-6 (k) and chemokine CXCL10 (f), MCP3 (j) levels were determined from bone marrow chimeric mice receiving Pld3–/–Pld4+/– (dark grey), Pld3+/–Pld4–/– (light grey), or Pld3–/–Pld4–/– (white) bone marrow 8 weeks earlier (Dotted line represents level of sensitivity of assay). (e-k) Mean and individual mouse values are shown. Statistics were determined using a two-tailed Student’s T test assuming equal variance. Experiment was performed once.
