Supplementary Figure 5: The 91-gene panel is relevant to discriminate gene expression profiles of normal B cell subsets and FL B cells in bulk gene expression datasets.

(a) Published gene expression microarray datasets for FACS-purified FL B cells (Green et al.), human LZ and DZ GC B cells (Victora et al.), and human circulating naïve and memory B cells (Seifert et al.), were downloaded and reanalyzed together. The top 500 variable genes on the combined dataset were used to generate a hierarchical clustering dendrogram (Euclidean distance, Ward agglomerative method) of all samples. (b) Only the probesets corresponding to the 91-gene panel analyzed by single-cell qPCR were retained for heatmap representation and hierarchical clustering (genes: Pearson correlation; samples: Euclidean distance; Ward agglomerative method). Of note, 21 out of the 91 genes were present in the top 500 variable genes.