Supplementary Figure 2: CD161⁺ T_reg cells are distinct from other CD161-expressing T cells.

(a) Representative example of the FACS sorting strategy for naive (orange), memory (yellow) and CD161⁺ (purple) T_reg cells. (b) Proportions of each T_reg subpopulation in healthy human donor peripheral blood (cumulative data from n = 10 donors); bars show mean + s.e.m. (c) Representative (top) and cumulative (bottom) expression of TCR Vα24-Jα18 and Vα7.2 in total CD4⁺ T cells and CD161⁺ T_reg cells. Both cell types show minimal expression of these invariant TCR chains. Data are from n = 3 independent experiments. (d) Contribution of the three highest (BV05, BV06 and BV07) and one of the lowest (BV19) TCRBV families to the overall TCR repertoire in naive, memory and CD161⁺ T_reg cells as well as CD161⁺ and total T_conv cells. Data are from n = 3 independent experiments; bars show mean + s.e.m. (e) Average percentage of TCR sequences either unique or shared among naive, memory and CD161⁺ T_reg cells, CD161⁺ and total T_conv cells (cumulative data from n = 3 experiments). *P < 0.01, **P < 0.001, ***P < 0.0001 by one-way ANOVA.